Outcome of oral implant treatment in partially edentulous jaws followed 20 years in clinical function

Background: Most long‐term follow‐up studies of implants in partially edentulous jaws present their outcomes as mean values of implant survival and follow‐up time, and few address the fate of the remaining teeth. Purpose: The aim of this study was to investigate the results of oral implant treatment in partially edentulous jaws after 20 years, and simultaneously to assess what happens to teeth present at the time of implant placement. Materials and Methods: Seventeen partially edentulous patients, of 27 originally treated individuals, were retrospectively reviewed after receiving implants from 1983 to 1985. The parameters studied were implant survival, prosthesis stability, marginal bone loss at teeth and implants, treatment complications, need for dental treatment, and patient’s satisfaction with the outcome. Results: The cumulative survival rate was 91%, when all 27 patients were assessed, that is, including the 10 dropouts. Of the 69 inserted and followed implants (Brånemark system®; Nobel Biocare AB, Göteborg, Sweden), six failed (8.7%) during the 20‐year period, four during the first decade, and the remaining two during the second. A majority (n=4) of the losses were due to implant fractures, two after 8 years, and two after 17 years. In all, 10 of the original fixed bridges being followed (n=24) remained in function during the entire investigation period, whereas 12 were exchanged for new constructions after an average of 7 years. The mean marginal bone loss at teeth was 0.7 mm, and at implants it was 1.0 mm. The major complication observed during the second decade was veneer material fractures, which occurred 14 times in six patients. Component loosening and abutment‐ and bridge‐locking screw fractures were the second most common problems seen, indicating material/component fatigue. Most patients were satisfied with their treatment and many mentioned that they did not think of the constructions as anything but a part of their own body. Conclusion: Over the decades, treatment of partially edentulous jaws with turned titanium implants seems to function well and to provide patients with good support for fixed short‐span bridge constructions.     

Do longer implants improve clinical outcome in immediate loading?

It is generally believed that longer implants (length >13 mm) have higher success rates than standard implants (length=13 mm). Few reports focus on long implants and none specifically address the clinical outcome of immediate loading (IL) of longer implants. This retrospective study was performed to compare the clinical outcomes of long and standard length implants. A total of 244 standard and 536 long implants were inserted and immediately loaded. The mean follow-up was 3 years. Only 4 of 780 implants (99.5%) were lost, and these 4 were all 13 mm long. No or reduced marginal bone loss was taken as an additional indicator of success to evaluate the effect of several factors on clinical outcome. Only 4 of the 244 13-mm-long implants were lost (98%), but this was statistically different from the survival rate of longer implants. Poor quality bone was related to increased marginal bone loss and thus a worse outcome in both groups. IL standard length implants have a high survival rate, but it is statistically worse than that of IL longer implants. Standard or longer implants are reliable devices for insertion in poor quality bone, although slightly higher bone resorption is to be expected.     

Septoclasts expressing epidermal fatty acid-binding protein (E-FABP,FABP5) in endochondral ossification

BackgroundLong-chain fatty acids (LCFAs) and retinoic acid (RA) are abundant in the growth plates (GPs) of long bones; however, their roles have not been elucidated. We observed that epidermal fatty acid-binding protein (E-FABP/FABP5) with a high affinity for both LCFAs and RA is exclusively expressed in the septoclasts located at the chondro-osseous junction (COJ) of the GP.HighlightsE-FABP expressed in septoclasts is involved in both LCFA metabolism and RA signaling as an intracellular transporter of both LCFAs and RA. Septoclasts with shortened cytoplasmic processes are associated with cartilage resorptive activity downregulation because of E-FABP deficiency or excess or deficiency of RA. In ontogeny, the septoclasts are differentiated from the pericytes and involved in the resorption of the uncalcified matrix of the cartilage templates in endochondral ossification.ConclusionSeptoclasts originate from pericytes and express E-FABP to play crucial roles in uncalcified matrix resorption by LCFA metabolism and RA signaling during endochondral ossification.     

A long-term follow-up study of non-submerged ITI implants in the treatment of totally edentulous jaws. Part I: Ten-year life table analysis of a prospective multicenter study with 1286 implants

In this prospective multicenter study, non-submerged ITI implants were followed in order to evaluate their long-term prognosis in fully edentulous jaws. A total of 1286 implants were inserted in 233 consecutive patients and, after a healing period of three to six months, the successfully integrated implants were restored with 163 overdentures and 95 fixed full-arch bridges. This prospective study not only calculated the 10-year cumulative survival and success rates for the 1286 implants by life table analysis, but also evaluated the actual survival and success rates for 498 implants after at least five years of functional loading. In addition, cumulative success rates were calculated for implant subgroups according to implant length and location. Additional analyses were performed to evaluate the estimated and actual survival and success rates of the implants in relation to various prosthetic rehabilitation techniques. The 10-year cumulative survival and success rates were 95.9% and 92.7%, respectively. The actual 5-year survival and success rates of the first 498 implants that were inserted were 97.7% and 95.0%, respectively. The analysis of implant subgroups showed slightly more favourable cumulative success rates for 12 mm long implants (93%), in comparison to 10 mm and 8 mm long implants (91.6% and 89.6%, respectively). The cumulative success rate for mandibular implants (approximately 94%) was also more favourable than that for maxillary implants (approximately 91%). Patients who were loaded with both maxillary and mandibular prostheses maintained success rates well above 90%; while only implants that were inserted to support maxillary overdentures that were retained by Dolder bars showed a success rate below 90%.     

长链非编码RNA肌动蛋白纤维相关蛋白1-反义RNA1在口腔鳞状细胞癌中的表达及其相关功能

目的 分析长链非编码RNA（lncRNA）肌动蛋白纤维相关蛋白1-反义RNA1（AFAP1-AS1）在口腔鳞状细胞癌（OSCC）中的表达及其对OSCC细胞生物行为学的影响,初步探讨其可能的作用机制。方法 采用实时荧光定量聚合酶链反应（qRT-PCR）检测OSCC患者（55例）癌组织、癌旁正常黏膜组织及人口腔鳞状细胞癌SCC25、人正常口腔角质细胞株（NOK）细胞中lncRNA AFAP1-AS1的表达,分析AFAP1-AS1与OSCC患者病理特征的相关性,通过Kaplan-Meier生存曲线分析AFAP1-AS1与患者预后的关系。AFAP1-AS1 siRNA转染SCC25细胞,细胞计数（CCK-8）及Transwell实验分别检测细胞增殖、迁移和侵袭的变化,蛋白质印迹（Western blot）检测侵袭相关蛋白、肌动蛋白纤维相关蛋白1（AFAP1）及Rho GTP酶家族成员蛋白的表达情况,免疫荧光染色检测细胞骨架肌动蛋白微丝结构的变化。结果 OSCC组织中AFAP1-AS1的表达高于癌旁正常黏膜组织,SCC25细胞中AFAP1-AS1的表达高于NOK细胞（P<0.001）。AFAP1-AS1的表达与OSCC的分化程度、TNM分期及淋巴结转移密切相关（P<0.05）,AFAP1-AS1高表达患者的生存率低于AFAP1-AS1低表达者（P<0.05）。AFAP1-AS1 siRNA转染后AFAP1-AS1的表达水平下调,SCC25细胞的增殖、迁移和侵袭能力降低,AFAP1、RhoA、Rac2、Rab10、RhoGDI和Pfn1的表达上调,RhoC的表达下调,细胞骨架中应力纤维丝减少,肌动蛋白完整性丢失。结论 lncRNA AFAP1-AS1在OSCC组织及SCC25细胞中高表达,与OSCC的发生进展及预后相关。下调AFAP1-AS1能够抑制OSCC的增殖、迁移和侵袭能力,其可能是通过调控肌动蛋白纤维丝的完整性实现的。     

利用数据库信息构建乳腺癌免疫关联lncRNAs风险评估模型

目的 筛选乳腺癌中免疫关联长链非编码RNA(lncRNA),并构建乳腺癌预后风险评估模型,探索预后相关因素。 方法 从UCSC Xena(https://xena.ucsc.edu/)、TCGA、immport(https://www.immport.org/home)官网分别下载乳腺癌患者的测序数据、临床信息以及免疫基因集,并将这些数据进行整理和清洗,最终得到乳腺癌免疫关联lncRNA表达矩阵及临床信息。利用单因素Cox和多因素Cox回归分析筛选出与预后相关的免疫关联 lncRNA,用于构建预后风险评分。根据风险评分的中位数,将患者分为高风险组和低风险组,利用Kaplan-Meier(K-M)生存分析、受试者工作特征曲线(ROC)分析及独立预后因素评估对模型进行评价,并将此模型联合其他临床因素构建列线图,对乳腺癌患者进行生存率预测。 结果 最终确定10个免疫关联 lncRNAs 用来构建风险评分模型;高风险组较低风险组预后差;风险评分可作为乳腺癌患者的独立预后因素;列线图的C指数(CI)为0.751,校准图显示预测值与实际观测值一致性较好。 结论 由10个免疫关联lncRNAs 组成的风险评分模型可用于评估乳腺癌患者的预后,由此建立的列线图可进一步预测乳腺癌患者的生存率。     

干扰长链编码RNA FOXCUT能抑制鼻咽癌细胞上皮间质转化及诱导线粒体损伤

目的 探讨干扰长链编码RNA FOXCUT对鼻咽癌细胞上皮间质转化及线粒体功能的影响。方法 RT-PCR检测50例鼻咽癌患者癌组织及癌旁组织和NP69、CNE1、CNE2、SUNE2、HER2和5-8F细胞株中FOXCUT表达水平;将shRNA FOXCUT转染至CNE1细胞,随机分组为Control组、shRNA-NC组和FOXCUT-shRNA3组。采用CCK8法和克隆形成实验检测细胞增殖;显微镜观察细胞形态;免疫荧光检测Vimentin+含量;试剂盒检测氧化应激标记物SOD、MDA、LDH的水平;流式检测线粒体膜电位的变化;Western blot法检测E-cad、N-cad、Vimentin、Bax、Bcl-2、caspase-3和c-Myc蛋白表达水平。结果 与癌旁组织相比,鼻咽癌组织中FOXCUT表达水平均升高（P<0.001）。与NP69细胞相比,CNE1、CNE2、SUNE2、HER2和5-8F细胞FOXCUT表达水平均升高（P<0.001）。与Control组相比较,FOXCUT-shRNA3组细胞增殖倍数及克隆形成率降低（P<0.001）,细胞形态呈短梭形、扁平型或圆形,细胞间的连接较为紧密,具有铺路石样特征;N-cad、Vimentin的表达水平降低,E-cad的表达水平升高（P<0.05）,Vimentin+含量降低（P<0.001）,MDA、LDH的含量明显升高（P<0.05）,SOD的活性明显降低（P<0.05）,红色荧光细胞向绿色荧光细胞转变较明显,绿色荧光细胞百分比增加,Bax/Bcl2、Cleaved cas3/cas3表达显著上升,c-Myc表达显著降低（P<0.001）。结论 干扰长链非编码RNA FOXCUT能够抑制鼻咽癌细胞增殖,减少上皮间质转化,增强氧化应激、降低膜电位,诱导CNE1细胞线粒体功能损伤,促进凋亡;干扰长链非编码RNA FOXCUT对CNE1细胞抑制效果显著,具有靶向治疗鼻咽癌的潜力。     

长链非编码RNAs与缺血性脑卒中

缺血性脑卒中是世界范围内导致死亡和残疾的主要原因之一.科学家们一直致力于阐明其中的细胞和分子病理生理过程.长链非编码RNAs(long non-coding RNAs,lncRNAs)在许多疾病中发挥重要作用.近年来,在缺血性脑卒中患者和脑缺血动物模型上发现了大量异常表达的lncRNAs.这些lncRNAs参与促进脑缺血后的细胞凋亡、血管形成、炎症反应和细胞死亡.上述发现可能有助于阐明lncRNAs在缺血性卒中的作用和机制.进一步探究lncRNAs在正常和病理条件下的功能和机制,可能有助于寻找缺血性卒中的生物标志物和新的治疗靶点.     

子痫前期孕妇血浆外泌体中LncRNA MALAT1和miR-206的表达水平及临床意义

目的 探究长链非编码RNA肺腺癌转移相关转录本1(LncRNA MALAT1)、微小RNA-206(miR-206)在子痫前期(PE)孕妇血浆外泌体中的表达水平及临床意义.方法 选取2017年1月至2019年12月在郑州市妇幼保健院住院分娩的PE孕妇42例为PE组,另选取同期在本院分娩的健康孕妇42例为对照组.根据入组PE患者病情严重程度分为轻度组22例,重度组20例.检测所有研究对象血浆外泌体LncRNA MALAT1、miR-206、血管内皮生长因子(VEGF)表达水平并进行组间比较.采用Pearson法分析PE孕妇血浆外泌体中LncRNA MALAT1、miR-206和VEGF的相关性,logistic回归模型分析PE发生的影响因素,采用受试者工作特征曲线(ROC)分析血浆外泌体LncRNA MALAT1、miR-206表达水平对PE发生的预测价值.结果 与对照组比较,PE组患者血浆外泌体miR-206水平明显升高,LncRNA MALAT、VEGF水平明显降低(P<0.05).LncRNA MALAT1、VEGF水平随PE患者病情加重有降低趋势,miR-206水平有升高趋势(P<0.05).高miR-206水平、低LncRNA MALAT1水平是PE发生的独立危险因素(P<0.05).LncRNA MALAT1、miR-206联合检测预测PE发生的曲线下面积为0.872,敏感度为92.86％.结论 PE患者血浆外泌体中LncRNA MALAT1呈低表达、miR-206呈高表达,两者均是PE发生的危险因素,二者联合检测对PE的诊断价值较高.     

串联质谱法在过氧化物酶体病筛查中的应用

目的：探讨串联质谱法检测极长链酰基肉碱（VLCAC）和溶血磷脂酰胆碱（LPC）在过氧化物酶体病筛查中的价值。方法：选取2017年1月至2021年3月以发育迟缓等神经系统异常就诊于上海市儿童医院,根据临床症状、磁共振成像和基因检测结果明确诊断为X-连锁肾上腺脑白质营养不良（X-ALD）患儿14例和脑肝肾综合征（ZS）患儿4例。另选取同年龄段体检儿童200名为健康对照组。使用含稳定同位素内标的溶剂萃取所有对象干血斑标本中的VLCAC和LPC,直接采用串联质谱法检测二十碳酰基肉碱（C20）、二十二碳酰基肉碱（C22）、二十四碳酰基肉碱（C24）、二十六碳酰基肉碱（C26）、二十碳溶血磷脂酰胆碱（C20:0-LPC）、二十二碳溶血磷脂酰胆碱（C22:0-LPC）、二十四碳溶血磷脂酰胆碱（C24:0-LPC）和二十六碳溶血磷脂酰胆碱（C26:0-LPC）水平,并计算C24/C20、C24/C22、C26/C20、C26/C22、C24:0-LPC/C20:0-LPC、C24:0-LPC/C22:0-LPC、C26:0-LPC/C20:0-LPC、C26:0-LPC/C22:0-LPC比值。采用Kruskal-Wallis H检验和Mann-WhitneyU检验比较各组间VLCAC和LPC各指标检测值及比值,采用偏最小二乘法和变量投影重要度权重评分分析各指标对判断疾病的贡献度。结果：除C24:0-LPC/C20:0-LPC外,所有指标和比值在各组间差异均有统计学意义（P<0.05或P<0.01）;X-ALD组与健康对照组、ZS组与健康对照组间,各指标有不同程度的差异,但X-ALD组与ZS组间差异无统计学意义（P>0.05）;偏最小二乘法分析显示X-ALD和ZS组与健康对照组能够完全分离,C26的变量投影重要度值最大。结论：串联质谱法检测VLCAC和LPC可作为过氧化物酶体病筛查的方法,其中C26或可作为诊断敏感指标。